Human Anti-ASGR Recombinant Antibody (TAB-1013CLV)
CAT#: TAB-1013CLV
This product is a recombinant human antibody that recognizes human ASGPR. The antibody was purified by affinity chromatography. This antibody can be used for FC, FRET and Internalization assay.
Tested Data
SDS-PAGE
Figure 1 Human Anti-ASGR Recombinant Antibody (TAB-1013CLV) in SDS-PAGE
SDS-PAGE analysis of TAB-1013CLV in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 275 kDa.
SEC-HPLC
Figure 2 Human Anti-ASGR Recombinant Antibody (TAB-1013CLV) in SEC-HPLC
SEC-HPLC analysis of TAB-1013CLV shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Human Anti-ASGR Recombinant Antibody (TAB-1013CLV) in DB
Dot Blot analysis of TAB-1013CLV was performed by coating with human ASGR1 protein (His tag).
TAB-1013CLV incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
WB
Figure 4 Human Anti-ASGR Recombinant Antibody (TAB-1013CLV) in WB
Western blot analysis of TAB-1013CLV was performed by loading human ASGR1 protein, His tag.
TAB-1013CLV incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L).
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 5 Human Anti-ASGR Recombinant Antibody (TAB-1013CLV) in ELISA
ELISA analysis of TAB-1013CLV was performed by coating with human ASGR1 protein (His tag). Then blocked with BSA and incubated with TAB-1013CLV. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- Extracellular domain (ECD) of human ASGPR HI
- Host Species
- Human
- Type
- Humanized IgG1, λ
- Specificity
- Human
- Species Reactivity
- Cross-react with cynomolgus monkey
- Applications
- FC, FRET, Internalization
- Related Disease
- Liver diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ASGR1; asialoglycoprotein receptor 1; HL-1; ASGPR; ASGPR1; CLEC4H
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An extended time of strong steadiness
The antibody was stored at -20°C for several months without losing any of its exceptional binding properties. Great for longer-term projects.
Effective in multiplex testing
used this antibody together with a few others in a multiplex test. There were no cross-reactivity issues, and the detection was precise and accurate.
Excellent customer service
The customer support team was very helpful in providing detailed product details and troubleshooting guidance.
Q&As
-
How should I handle antibodies during extended getaways or holidays?
A: Store antibodies according to manufacturer recommendations for short-term conditions, including aliquoting if necessary.
-
What are the best washing conditions for antibodies?
A: Washing buffers, typically PBS or TBS with Tween-20, should effectively remove unbound antibodies without dislodging immobilized antigens or complexes.
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Does the provider supply datasheets and a Certificate of Analysis (CoA)?
A: Reputable vendors provide datasheets and Certificates of Analysis (CoAs) that detail antibody specifications, validations, and recommended protocols.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-1013CLV, RRID: AB_3111808)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ASGR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1013CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1013CLV-S(P)) | ELISA | Humanized scFv |
| TAB-1014CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1014CLV-S(P)) | ELISA | Humanized scFv |
| TAB-1015CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1015CLV-S(P)) | ELISA | Humanized scFv |
| TAB-1016CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1016CLV-S(P)) | ELISA | Humanized scFv |
| TAB-1017CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1017CLV-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1019CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1019CLV-S(P)) | ELISA | Human scFv |
| TAB-1020CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1020CLV-S(P)) | ELISA | Human scFv |
| TAB-1021CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1021CLV-S(P)) | ELISA | Human scFv |
| TAB-1022CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1022CLV-S(P)) | ELISA | Human scFv |
| TAB-1023CLV-S(P) | Human Anti-ASGR Recombinant Antibody; scFv Fragment (TAB-1023CLV-S(P)) | ELISA | Human scFv |
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